Toxicity effects of Diethyl Phtalate (DEP) on the freshwater fish Nile Tilapia ( Oreochromis niloticus)/ John Pierre C. Donadillo, Deo Angelo B. Navarro, Joshua L. Rosete, and Psalm Danielle S. Torres .--

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Donadillo, John Pierre C [author ]

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Material type: Text

TextPublication details: Manila: Technological University of the Philippines, 2024.Description: ix, 151pages: 29cm. +1 CD-ROM (4 3/4in.)Content type:

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BTH GF 41 D66 2024

Dissertation note: College of Science .-- Bachelor of Science in Environmental Science: Technological University of the Philippines, 2024. Summary: Abstract: Diethyl phthalate (DEP) is considered an emerging synthetic chemical pollutant utilized as a plasticizer for personal care, insecticides, plastic packaging, and other plastic products. Detecting these chemicals is important to understand the toxicity effect of DEP. This study aimed to evaluate the median lethal concentration (LC50) of DEP on Nile Tilapia (Oreochromis niloticus) fingerlings and assess the behavioral and morphological changes to DEP and the presence of DEP in Pampanga River in San Miguel, Bulacan. The acute toxicity was tested to determine the LC50 of the DEP on the Nile Tilapia (Oreochromis niloticus) fingerlings by static bioassay method using probit analysis. Fingerlings were exposed to different concentrations (0, 25, 35, 50, and 75 ppm) of DEP for 96 hours. The lethal concentration 50 value was 48.073 mg/L with a 95% confidence limit. The Pampanga River has a < 1 μg/L presence of DEP. Fingerlings were exposed to sub-lethal concentration (1/10 of LC50) that allowed monitoring of their behavioral and morphological changes within 7 days. The toxicity effects of DEP on Nile Tilapia (Oreochromis niloticus) are evident even at sub-lethal concentrations to have behavioral and morphological changes. Fingerlings showed sluggishness, loss of equilibrium, abnormal movement, and changes in dark skin pigmentation on the dorsal surface. Fingerlings exposed to DEP showed significantly reduced growth compared to the control group, with a 46% difference in weight and an 11% difference in length. The water quality parameters of aquariums were aligned with the BFAR guidelines for the growth of Nile Tilapia fingerlings. This includes pH ranging from (7.71 to 8.16), temperature (27.8 °C to 28.1 °C), TDS (84 mg/L to 113 mg/L), salinity (84 mg/L to 113 mg/L), and DO (5.1 mg/L to 7.1 mg/L). This encourages people to be aware of the adverse effects of pollutants in the Pampanga River, San Miguel, Calumpit, Bulacan, Philippines.

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Item type	Current library	Shelving location	Call number	Copy number	Status	Notes	Date due	Barcode
Bachelor's Thesis COS	TUP Manila Library	Thesis Section-2nd floor	BTH GF 41 D66 2024 (Browse shelf(Opens below))	c.1.	Not for loan	For library use only		BTH0005249

Thesis (undergraduate)

College of Science .-- Bachelor of Science in Environmental Science: Technological University of the Philippines, 2024.

Includes bibliography:

Abstract: Diethyl phthalate (DEP) is considered an emerging synthetic chemical
pollutant utilized as a plasticizer for personal care, insecticides, plastic packaging, and
other plastic products. Detecting these chemicals is important to understand the toxicity
effect of DEP. This study aimed to evaluate the median lethal concentration (LC50) of
DEP on Nile Tilapia (Oreochromis niloticus) fingerlings and assess the behavioral and
morphological changes to DEP and the presence of DEP in Pampanga River in San
Miguel, Bulacan. The acute toxicity was tested to determine the LC50 of the DEP on the
Nile Tilapia (Oreochromis niloticus) fingerlings by static bioassay method using probit
analysis. Fingerlings were exposed to different concentrations (0, 25, 35, 50, and 75 ppm)
of DEP for 96 hours. The lethal concentration 50 value was 48.073 mg/L with a 95%
confidence limit. The Pampanga River has a < 1 μg/L presence of DEP. Fingerlings were
exposed to sub-lethal concentration (1/10 of LC50) that allowed monitoring of their
behavioral and morphological changes within 7 days. The toxicity effects of DEP on Nile
Tilapia (Oreochromis niloticus) are evident even at sub-lethal concentrations to have
behavioral and morphological changes. Fingerlings showed sluggishness, loss of
equilibrium, abnormal movement, and changes in dark skin pigmentation on the dorsal
surface. Fingerlings exposed to DEP showed significantly reduced growth compared to
the control group, with a 46% difference in weight and an 11% difference in length. The
water quality parameters of aquariums were aligned with the BFAR guidelines for the
growth of Nile Tilapia fingerlings. This includes pH ranging from (7.71 to 8.16),
temperature (27.8 °C to 28.1 °C), TDS (84 mg/L to 113 mg/L), salinity (84 mg/L to 113
mg/L), and DO (5.1 mg/L to 7.1 mg/L). This encourages people to be aware of the
adverse effects of pollutants in the Pampanga River, San Miguel, Calumpit, Bulacan,
Philippines.

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