Arbois, Steve Ashbey A.

Phytochemical screening, cytotoxicity, and anti oxidant properties of Kalumpit ( Terminalia microcarpa) seeds extract/ Steve Ashbey A. Arbois, Reynan D. Llemit, Karl Francis G. Ropan, and Gian Angelo B. Valderrama.-- - Manila: Technological University of the Philippines, 2023. - 130pages: 29cm.

Thesis (undergraduate)

College of Science.--

Includes bibliography:

This study determined the phytochemical constituents, antioxidant property, and
toxicity of Terminalia microcarpa (kalumpit) seed extracts obtained using two different
solvents: ethanol and ethyl acetate. The study employed the Brine Shrimp Toxicity Test to
measure the toxicity and the Folin-Ciocalteu Assay to determine the total phenolics content
indicative of antioxidant potential. Results demonstrated that both solvents extracted
bioactive compounds exhibiting a dose-dependent toxicity. Ethyl acetate extracts revealed
more pronounced toxicity than ethanol extracts. However, ethanol was significantly more
efficient in extracting antioxidant compounds, with an average total phenolics content of
315.67 mg GAE per mL, compared to 27.67 mg GAE per mL for ethyl acetate. The study
concludes that ethanol is a more suitable solvent for extracting antioxidants from
Terminalia microcarpa seeds due to its higher yield and lesser toxicity. It is advisable to
conduct further research to identify the specific bioactive compounds present in the
extracts, establish their correlation with the observed properties, and evaluate the safety of
these extracts for potential applications.
Keyword: Terminalia microcarpa, Phytochemical screening, Antioxidant activity, and
Cytotoxicity


Terminalia microcarpa ( kalumpit)
Brine Shrimp toxicity

BTH RB 37 / A73 2023