Del Carmen, Kristine Joy C.  <br/><br/>
Extraction and characterization of crude anthraquinones from duhat (Syzygium cumini) leaves and their anthelmintic activity against Ascaris suum eggs/  
Kristine Joy C. Del Carmen, Omelhair O. Gonaranao, and Richard Timothy F. Baluyot .-- 
 - Manila:  Technological University of the Philippines,   2024. 
 - 44pages:  29cm.   +1 CD-ROM (4 3/4in.) 
<br/><br/> Thesis (undergraduate) 
<br/><br/>College of Science.-- 
<br/><br/>Includes bibliography.  
<br/><br/> Ascaris suum eggs provide substantial health risks to both humans and animals. Their<br/>resilience makes it difficult for traditional approaches to eliminate their population. Studies<br/>have used phytochemicals to eradicate them, with anthraquinone being one example. This<br/>study investigated the anthelmintic action of anthraquinones isolated from duhat (Syzygium<br/>cumini) leaves against Ascaris suum eggs. The duhat leaves were extracted using 60%<br/>ethanol. The phytochemical analysis confirmed that duhat leaves extract contains bioactive<br/><br/>compounds. The duhat leaves extracted were purified with the Alcohol/Salt Aqueous Two-<br/>Phase System (ATP), which included 1-propanol and ammonium sulphate. The<br/><br/>characterization process was employed to confirm and identify the extract. The absorption<br/>peak at 280 nm confirmed the presence of anthraquinone using UV-Vis Spectroscopy.<br/>Fourier Transform Infrared Spectroscopy identified different peaks at 1409cm-1<br/>and<br/><br/>1016.49 cm-1<br/><br/>corresponds to conjugated C=C bond (aromatic), and CO bond. The presence<br/>of -OH bonds indicates that the solvent was not removed. The crude anthraquinone isolated<br/>from duhat leaves was tested for anti-helmintic efficacy against Ascaris suum at three<br/>different concentrations (25%, 50%, and 100%). The ANOVA showed that the<br/>concentrations of 25% and 100% of anthraquinones duhat leaves extract have less than<br/>0.05 significance difference. The different amounts of duhat extracts had similar effects on<br/>the antibiotic while dissolving Ascaris suum eggs. The results indicate that crude<br/>anthraquinones derived from duhat leaves extract are potential anthelmintic agent against<br/>Ascaris suum. It was also revealed that the anthraquinone present in the extract was not<br/>effectively purified using the ATPS method<br/>————————————————————————————————————<br/>Keywords: anthelmintic, anthraquinones, ANOVA, Ascaris suum, ATPs, duhat,<br/>phytochemical 
<br/><br/><label>LC Class. No.: </label>BTH RB 37  / D45 2024