Green synthesis of zinc oxide nanoparticles using butterfly pea (Clitoria ternatea L.) leaf extract and evaluation of their antibacterial properties/
Junnelyn M. Bulactial, Elirose Kyll M. Flores, Nickelyn P. Martinez, Brin Mae M. Uy .--
- Manila: Technological University of the Philippines, 2024.
- xii, 96pages: 29cm. +1 CD-ROM (4 3/4in.)
Thesis (undergraduate)
College of Science.--
Includes bibliography:
The extensive use of antibiotics has accelerated the emergence of antibiotic-resistant bacteria, presenting a major threat to public health. This study explored an alternative antimicrobial strategy through the green synthesis of Zinc oxide Nanoparticles (ZnO NPs) using Clitoria ternatea L. leaf extract. The calcined zinc oxide nanoparticles yielded 48.7%. ZnO NPs were characterized using several techniques. UV-visible spectrophotometry showed an absorbance peak at 304 nm, confirming ZnO NP formation. FTIR analysis revealed peaks at 1435 cm−1 (C=O), 1146 cm−1 (C-O), 880 cm−1 (C-H), and 419 cm-1
(Zn-O). SEM and TEM images indicated hexagonal and spherical morphologies with an average size of 41 nm. EDS analysis confirmed the elemental composition with zinc and oxygen accounting for 30.49% and 47.56%, respectively. XRD patterns confirmed the crystalline nature of the nanoparticles. Despite successful synthesis and detailed characterization, the antibacterial activity of ZnO NPs, assessed using the Disc Diffusion Method, showed no inhibitory effect against Gram-positive Staphylococcus aureus and Gram-negative Salmonella typhi. These findings suggested that further optimization of the synthesis process or additional functionalization of ZnO NPs might be necessary to enhance their antimicrobial properties. While the potential of green synthesis methods was evident, the challenges in developing effective nanoparticle-based antibacterial agents were also underscored. Keywords: Clitoria ternatea L., ZnO NPs, antibacterial efficacy, Disc diffusion method, Staphylococcus aureus