Cuaresma, Caylah C.

Purple heart (tradescantia pallida) leaves anthocyanin extract as a natural dye powder/ Caylah C. Cuaresma, Andrea Shine C. De Jesus, and Germaine Ashly H. Moratilla.-- - Manila: Technological University of the Philippines, 2025. - xii, 151pages: 29cm.

Bachelor's thesis


College Of Science.--


Includes bibliographic references and index.

Anthocyanins are used as natural pigments in various industries, due to the color palette
they can produce from red to blue and purple. However, their stability is influenced by
parameters such as pH, light, and temperature. This study examined the potential of
anthocyanin pigments extracted from Purple Heart (Tradescantia pallida) leaves as a
natural dye powder to provide an eco-friendly alternative to synthetic dyes. Anthocyanins
were successfully extracted using 30% ethanol with 5% citric acid. Subsequent purification
via liquid-liquid extraction and dialysis removed impurities, confirmed by a reduction in
absorbance and a single spot (Rf = 0.525) using 6:3:1 (hexane:ethanol:ethyl acetate) solvent
system in Thin Layer Chromatography. The purified extract was converted into a powder
through freeze-drying, yielding an average of 0.782% and a moisture content of 0.34%.
Characterization through pH-dependent color changes and Fourier-Transform Infrared
(FTIR) spectroscopy identified petunidin, and malvidin derivatives, with prominent O-H,
C=O, C-H and C-O functional groups. Scanning Electron Microscopy with Energy
Dispersive Spectroscopy (SEM-EDS) revealed polydisperse particles and confirmed the

organic elemental composition of the dye powder. Furthermore, Ultraviolet-Visible (UV-
Vis) and FTIR analyses showed progressive degradation under increasing temperatures

(30°C, 40°C, 60°C, 80°C) and light exposure, with a noticeable decrease in absorbance and
peak intensities. When applied to pre-mordanted cotton fabrics using Copper sulfate
(CuSO4) as mordant, the dye exhibited minimal staining (grayscale 4.5) but poor
colorfastness to laundering, with significant color change (grayscale 1-1.5).


Anthocyanins
Freeze-drying
Dialysis

BTH RB 37 / C83 2025