Phytochemical screening, cytotoxicity, and anti oxidant properties of Kalumpit ( Terminalia microcarpa) seeds extract/ Steve Ashbey A. Arbois, Reynan D. Llemit, Karl Francis G. Ropan, and Gian Angelo B. Valderrama.--

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Arbois, Steve Ashbey A [author ]

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Material type: Text

TextPublication details: Manila: Technological University of the Philippines, 2023.Description: 130pages: 29cmContent type:

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BTH RB 37 A73 2023

Dissertation note: College of Science.-- Bachelor of Applied Science in Laboratory Technology: Technological University of the Philippines, 2023. Summary: This study determined the phytochemical constituents, antioxidant property, and toxicity of Terminalia microcarpa (kalumpit) seed extracts obtained using two different solvents: ethanol and ethyl acetate. The study employed the Brine Shrimp Toxicity Test to measure the toxicity and the Folin-Ciocalteu Assay to determine the total phenolics content indicative of antioxidant potential. Results demonstrated that both solvents extracted bioactive compounds exhibiting a dose-dependent toxicity. Ethyl acetate extracts revealed more pronounced toxicity than ethanol extracts. However, ethanol was significantly more efficient in extracting antioxidant compounds, with an average total phenolics content of 315.67 mg GAE per mL, compared to 27.67 mg GAE per mL for ethyl acetate. The study concludes that ethanol is a more suitable solvent for extracting antioxidants from Terminalia microcarpa seeds due to its higher yield and lesser toxicity. It is advisable to conduct further research to identify the specific bioactive compounds present in the extracts, establish their correlation with the observed properties, and evaluate the safety of these extracts for potential applications. Keyword: Terminalia microcarpa, Phytochemical screening, Antioxidant activity, and Cytotoxicity

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Item type	Current library	Shelving location	Call number	Copy number	Status	Notes	Date due	Barcode
Bachelor's Thesis COS	TUP Manila Library	Thesis Section-2nd floor	BTH RB 37 A73 2023 (Browse shelf(Opens below))	c.1.	Not for loan	For library use only		BTH0003302

Thesis (undergraduate)

College of Science.-- Bachelor of Applied Science in Laboratory Technology: Technological University of the Philippines, 2023.

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This study determined the phytochemical constituents, antioxidant property, and
toxicity of Terminalia microcarpa (kalumpit) seed extracts obtained using two different
solvents: ethanol and ethyl acetate. The study employed the Brine Shrimp Toxicity Test to
measure the toxicity and the Folin-Ciocalteu Assay to determine the total phenolics content
indicative of antioxidant potential. Results demonstrated that both solvents extracted
bioactive compounds exhibiting a dose-dependent toxicity. Ethyl acetate extracts revealed
more pronounced toxicity than ethanol extracts. However, ethanol was significantly more
efficient in extracting antioxidant compounds, with an average total phenolics content of
315.67 mg GAE per mL, compared to 27.67 mg GAE per mL for ethyl acetate. The study
concludes that ethanol is a more suitable solvent for extracting antioxidants from
Terminalia microcarpa seeds due to its higher yield and lesser toxicity. It is advisable to
conduct further research to identify the specific bioactive compounds present in the
extracts, establish their correlation with the observed properties, and evaluate the safety of
these extracts for potential applications.
Keyword: Terminalia microcarpa, Phytochemical screening, Antioxidant activity, and
Cytotoxicity

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