Green synthesis of zinc oxide nanoparticles using butterfly pea (Clitoria ternatea L.) leaf extract and evaluation of their antibacterial properties/ Junnelyn M. Bulactial, Elirose Kyll M. Flores, Nickelyn P. Martinez, Brin Mae M. Uy .--

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Bulactial, Junnelyn M [author ]

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TextPublication details: Manila: Technological University of the Philippines, 2024.Description: xii, 96pages: 29cm. +1 CD-ROM (4 3/4in.)Content type:

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BTH RB 37 B85 2024

Dissertation note: College of Science.-- Bachelor of Applied Science in Laboratory Technology: Technological University of the Philippines, 2024. Summary: The extensive use of antibiotics has accelerated the emergence of antibiotic-resistant bacteria, presenting a major threat to public health. This study explored an alternative antimicrobial strategy through the green synthesis of Zinc oxide Nanoparticles (ZnO NPs) using Clitoria ternatea L. leaf extract. The calcined zinc oxide nanoparticles yielded 48.7%. ZnO NPs were characterized using several techniques. UV-visible spectrophotometry showed an absorbance peak at 304 nm, confirming ZnO NP formation. FTIR analysis revealed peaks at 1435 cm−1 (C=O), 1146 cm−1 (C-O), 880 cm−1 (C-H), and 419 cm-1 (Zn-O). SEM and TEM images indicated hexagonal and spherical morphologies with an average size of 41 nm. EDS analysis confirmed the elemental composition with zinc and oxygen accounting for 30.49% and 47.56%, respectively. XRD patterns confirmed the crystalline nature of the nanoparticles. Despite successful synthesis and detailed characterization, the antibacterial activity of ZnO NPs, assessed using the Disc Diffusion Method, showed no inhibitory effect against Gram-positive Staphylococcus aureus and Gram-negative Salmonella typhi. These findings suggested that further optimization of the synthesis process or additional functionalization of ZnO NPs might be necessary to enhance their antimicrobial properties. While the potential of green synthesis methods was evident, the challenges in developing effective nanoparticle-based antibacterial agents were also underscored. Keywords: Clitoria ternatea L., ZnO NPs, antibacterial efficacy, Disc diffusion method, Staphylococcus aureus

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Item type	Current library	Shelving location	Call number	Copy number	Status	Notes	Date due	Barcode
Bachelor's Thesis COS	TUP Manila Library	Thesis Section-2nd floor	BTH RB 37 B85 2024 (Browse shelf(Opens below))	c.1.	Not for loan	For library use only		BTH0005000

Thesis (undergraduate)

College of Science.-- Bachelor of Applied Science in Laboratory Technology: Technological University of the Philippines, 2024.

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The extensive use of antibiotics has accelerated the emergence of antibiotic-resistant
bacteria, presenting a major threat to public health. This study explored an alternative
antimicrobial strategy through the green synthesis of Zinc oxide Nanoparticles (ZnO
NPs) using Clitoria ternatea L. leaf extract. The calcined zinc oxide nanoparticles yielded
48.7%. ZnO NPs were characterized using several techniques. UV-visible
spectrophotometry showed an absorbance peak at 304 nm, confirming ZnO NP
formation. FTIR analysis revealed peaks at 1435 cm−1 (C=O), 1146 cm−1 (C-O), 880 cm−1
(C-H), and 419 cm-1

(Zn-O). SEM and TEM images indicated hexagonal and spherical
morphologies with an average size of 41 nm. EDS analysis confirmed the elemental
composition with zinc and oxygen accounting for 30.49% and 47.56%, respectively.
XRD patterns confirmed the crystalline nature of the nanoparticles. Despite successful
synthesis and detailed characterization, the antibacterial activity of ZnO NPs, assessed
using the Disc Diffusion Method, showed no inhibitory effect against Gram-positive
Staphylococcus aureus and Gram-negative Salmonella typhi. These findings suggested
that further optimization of the synthesis process or additional functionalization of ZnO
NPs might be necessary to enhance their antimicrobial properties. While the potential of
green synthesis methods was evident, the challenges in developing effective
nanoparticle-based antibacterial agents were also underscored.
Keywords: Clitoria ternatea L., ZnO NPs, antibacterial efficacy, Disc diffusion method,
Staphylococcus aureus

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